Chemical Sensing Using Indicator Dyes

نویسنده

  • Otto S. Wolfbeis
چکیده

T h i s chapter describes the des ign, f a b r i c a t i o n , a n d propert ies of mater ia ls that respond to the presence of a chemical species by a change i n their opt ical properties. Ideally, such effects are reversible. They may be detected by conventional methods of absorpt ion, reflection, or luminescence spectrometry, a n d appl ied i n various formats such as test strips and disposable tests, but preferably by m a k i n g use of optical waveguides i n c l u d i n g optical fibers, integrated optics, capi l lary type devices, and the l ike . Specifically, we describe the design and use of appropriate indicator dyes, polymers , and addit ives, w i t h a part icular focus on materials for sensing p H , oxygen, carbon dioxide, a m m o n i a , and certain ions. These materials (or their solutions i n an appropriate solvent) may be deposited on various supports i n c l u d i n g simple plastic strips (e.g.. by spin-coat ing) , on fibers (e.g., by dip coating), inside porous materials (e.g., by soaking) , on integrated waveguides or walls of disposable cuvettes (e.g., by spreading the solutions as th in films), inside capil laries (e.g., by passing the solution through the capi l lary) , on the bottom of microtitre plates, or inside disposable vials.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Pattern-based sensing of nucleotides in aqueous solution with a multicomponent indicator displacement assay.

A multicomponent indicator displacement assay (MIDA) based on an organometallic receptor and three dyes can be used for the identification and quantification of nucleotides in aqueous solution at neutral pH.

متن کامل

Luminescent sensing and imaging of oxygen: Fierce competition to the Clark electrode

Luminescence-based sensing schemes for oxygen have experienced a fast growth and are in the process of replacing the Clark electrode in many fields. Unlike electrodes, sensing is not limited to point measurements via fiber optic microsensors, but includes additional features such as planar sensing, imaging, and intracellular assays using nanosized sensor particles. In this essay, I review and d...

متن کامل

Synthesis of squaraine dyes under mild conditions: applications for labelling and sensing of biomolecules.

We report the synthesis of squaraine dyes under mild conditions by carbodiimide activation of squaric acid or semi-squaraine dyes. Despite low yields when the reaction was carried out in solution, these conditions were successfully applied to efficient peptide labelling on resin and nucleic acid sensing in solution.

متن کامل

Nanoscale porosity in pigments for chemical sensing.

Porous pigments in which chemically responsive dyes have been immobilized in a matrix of organically modified siloxanes (ormosils) have been prepared and characterized by AFM, TEM, EDS, and optical analysis. In typical chemical sensing applications, an array of 36 different porous ormosil pigments are deposited on polyethylene terephthalate (PET) film.

متن کامل

DNA-hosted Hoechst dyes: application for label-free fluorescent monitoring of endonuclease activity and inhibition.

A simple and facile approach was developed for monitoring EcoRI endonuclease activity and inhibition, in which a hairpin-like DNA containing restriction cutting site for EcoRI endonuclease acts as the sensing element and Hoechst dyes as the signal indicator in a label-free format.

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2011